2024 Volume No 48 – pages 1-16
Title: Cellular response to short-time mechanical stimuli: mediating Ca2+ influx via PIEZO1 |
Authors: Fuan Wang, Hongkun Chen, Zhongyuan He, Jianfeng Li, Zhengya Zhu, Tao Tang, Junhong Li, Jiaxiang Zhou, Qiuxiao Tan, Zhen Li, Martin J. Stoddart, Xizhe Liu, Manman Gao, Zhiyu Zhou, Shaoyu Liu |
Address: Xizhe Liu, Guangdong Provincial Key Laboratory of Orthopedics and Traumatology, The First Affiliated Hospital of Sun Yat-sen University, 510060 Guangzhou, Guangdong, China. Manman Gao, Department of Orthopedics, Fuzhou Second Hospital, 350007 Fuzhou, Fujian, China. Zhiyu Zhou, Department of Orthopaedic Surgery, Innovation Platform of Regeneration and Repair of Spinal Cord and Nerve Injury, The Seventh Affiliated Hospital, Sun Yat-sen University, 510275 Shenzhen, Guangdong, China; Guangdong Provincial Key Laboratory of Orthopedics and Traumatology, The First Affiliated Hospital of Sun Yat-sen University, 510060 Guangzhou, Guangdong, China |
E-mail: liuxizhe at mail.sysu.edu.cn; gaomanm at mail2.sysu.edu.cn; zhouzhy23 at mail.sysu.edu.cn |
Abstract: Physical activity shows a positive correlation with overall health, and vigorous intermittent lifestyle physical activity (VILPA) similarly offers advantages in reducing the risk of all-cause mortality. Might the short-time mechanical stimuli be discernible to cells, eliciting commensurate physiological responses? The study's objective was to investigate the cellular response to short-time mechanical stimuli. Human umbilical cord-derived mesenchymal stem cells (hUCMSCs), isolated and thoroughly characterized, were subjected to various stimuli, including activation and mechanical stretching, with Ca2+ influx assessed through alterations in fluorescence intensity. Further validation of these findings was confirmed through short hairpin RNA (shRNA) and inhibitors. In addition, a comprehensive examination of PIEZO1 alterations was conducted through quantitative real-time polymerase chain reaction (qRT-PCR) and western blot (WB) techniques. The results shown different frequencies of stretching stimulation and durations induced varying degrees of Ca2+ influx. The most substantial increase occurred within 2–3 minutes in the group subjected to 0.5 Hz stretching for 2 minutes (p < 0.05). Stretching at 0.5 Hz resulted in significant elevation in PIEZO1 mRNA expression at 15 minutes and 1 hour. Additionally, stretching cause a gradual rise in PIEZO1 protein levels, with a notable peak observed at 2 hours. In conclusion, cells primarily sense short-time mechanical stimuli through PIEZO1, predominantly mediated by regulated Ca2+ influx. This underscores PIEZO1's crucial role in cellular responsiveness to transient mechanical cues, advancing our understanding of mechanosensory mechanisms in cellular physiology. |
Keywords: Vigorous intermittent lifestyle physical activity, short-time mechanical stimuli, PIEZO1, Ca2+ influx, Yoda1. |
Publication date: 16th July 2024 |
Copyright policy: © 2024 The Author(s). Published by Forum Multimedia Publishing, LLC. This article is distributed in accordance with Creative Commons Attribution Licence (http://creativecommons.org/licenses/by/4.0/). |
Article download: Pages 1-16 (PDF file) |
