2025 Volume No 49 – pages 21-31
Title: Experimental study on the main influencing factors of induced membrane-guided spontaneous osteogenesis |
Authors: J Liu, QD Yin, FY Bu, XM Chen |
Address: Department of Orthopaedics, Wuxi Ninth People’s Hospital Affiliated to Soochow University, 214063 Wuxi, Jiangsu, China |
E-mail: bofanyuyqd at 163.com; mingyin031 at 163.com |
Abstract: Background: Induced membrane-guided spontaneous osteogenesis (IMGSO) has been experimentally validated, but there is a lack of research on its cause and influencing factors. The aim of this study was to investigate the main influencing factors of IMGSO. Methods: Seventy-two adult Sprague-Dawley rats with 10- or 15-mm femur defects were established and randomly divided into 6 groups (n = 12). Except for the control group (Group B4), all the defects were filled with vancomycin-loaded polymethyl methacrylate bone cement spacers. Size-matched spacers for Groups A1–A2 and B1, larger spacers for Group B2, and bone ends sealed for Group B3. The osteogenic activities of different parts of the induced membrane (IM) in Groups A1–A2 and spontaneous osteogenic manifestations in Groups B1–B4 were observed. Results: At 5 weeks, the number of bone mesenchymal stem cells and the protein and mRNA expression levels of bone morphogenic protein-2 (BMP-2), transforming growth factor-β1 (TGF-β1) and vascular endothelial growth factor (VEGF) in the proximal and distal parts of the IMs were similar and greater than those in the middle part (p < 0.05). At 12 weeks, Group B1 had more new bone formation along the IM originated from the bone end than Group B2 (p < 0.05), while Groups B3 and B4 had no new bone, only bone resorption and bone atrophy. Conclusions: The osteogenic activities of different parts of the IMs vary, with the strongest activity in the IM near the bone end. Bone marrow overflowing of the bone end enhances the osteogenic activities of the IMs, resulting in IMGSO, and is the key factor. Another main influencing factor of IMGSO is the maintenance of an appropriate membrane size. |
Keywords: Bone defect, induced membrane, membrane-guided bone regeneration, bone cement, new bone formation. |
Publication date: 14th February 2025 |
Copyright policy: © 2025 The Author(s). Published by Forum Multimedia Publishing, LLC. This article is distributed in accordance with Creative Commons Attribution Licence (http://creativecommons.org/licenses/by/4.0/). |
Article download: Pages 21-31 (PDF file) |
