2025 Volume No 49 – pages 35-54
Title: Electrospun bioactive poly(ε-caprolactone) nanofibrous scaffolds incorporated with natural decellularized bone extracellular matrix for bone regeneration |
Authors: M Zhang, Q Zhou, QS Dong, J Zhang, X Zhou, H Huang, JC Bao, HJ Shan, FR Sun, LL Li |
Address: Department of Orthopaedic Surgery, The Second Hospital of Nanjing, 210003 Nanjing, Jiangsu, China |
E-mail: lianglianglinj at 163.com |
Abstract: Background: Cell-free bone tissue scaffolds show significant potential for bone repair applications. This study aimed to enhance the bone regeneration performance of synthetic poly(ε-caprolactone) (PCL)-based nanofibrous scaffolds by incorporating natural decellularized bone extracellular matrix (dB-ECM) into the scaffold. Methods: The PCL/dB-ECM nanofibrous scaffolds were fabricated by electrospinning. A series of in vitro and in vivo experiments were conducted to verify the biocompatibility of the PCL/dB-ECM nanofibrous scaffolds, as well as their ability to promote osteogenic differentiation of rabbit bone mesenchymal stem cells (rBMSCs) and to repair bone defects. Results: The incorporation of dB-ECM significantly enhanced the bioactivity of the PCL-based scaffold, yielding superior rBMSCs attachment and proliferation, cytoskeleton extension, and osteogenic differentiation. Furthermore, in vivo assays revealed that the PCL/dB-ECM (2:1) scaffold facilitated new bone formation, with improved trabecular structures and better integration with the surrounding tissues. Conclusions: The PCL/dB-ECM (2:1) nanofibrous scaffold presents a promising strategy for bone defect repair, offering enhanced bioactivity and effective integration with host tissues. |
Keywords: Bone regeneration, nanofibrous scaffold, decellularized extracellular matrix, poly(ε-caprolactone), electrospinning. |
Publication date: 14th February 2025 |
Copyright policy: © 2025 The Author(s). Published by Forum Multimedia Publishing, LLC. This article is distributed in accordance with Creative Commons Attribution Licence (http://creativecommons.org/licenses/by/4.0/). |
Article download: Pages 35-54 (PDF file) |
